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位置: 首页> 品牌> Sigma-Aldrich> 单克隆抗-β-连环蛋白

Anti-β-Catenin-Cy3 antibody, Mouse monoclonal

单克隆抗-β-连环蛋白

品牌
Sigma-Aldrich
货号
C7738
规格纯度
clone 15B8, purified from hybridoma cell culture
价格
4731.57 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-β-Catenin-Cy3 antibody, Mouse monoclonal
Monoclonal Anti-β-Catenin
单克隆抗-β-连环蛋白
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Anti-β-Catenin-Cy3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant chicken catenin. β-catenin is 92 kDa. β-catenin protein is composed of a series of protein-protein interaction motifs that allow it to function as a scaffold. The N-terminus domain contains the binding site for β-catenin as well as phosphorylation sites recognized by Glycogen synthase kinase 3 beta (GSK3β) and the C-terminus contains the transcriptional activation domain and binding site for Teashirt.
The gene β-catenin (CTNNB1) is mapped to human chromosome 3p21. The protein localizes to the cytoplasm and the nucleus. β-catenin is also present at the centrosomes.

特异性

特异性识别哺乳动物和禽类β-连环蛋白。不与斑珠蛋白(γ-连环蛋白)发生交叉反应。

免疫原

重组鸡β-连环蛋白。

应用

Suitable for direct immunofluorescence at a dilution of 1:100 using methanol/acetone-fixed bovine MDBK/MCF-7 cells. The antiobody has been used for western blot analysis in mouse embryonic stem cells.
Anti-β-Catenin-Cy3 antibody has been used in

  • direct immunofluorescence
  • western blotting
  • staining
  • immunohistochemistry

包装

200 μL in glass insert

生化/生理作用

Catenin (cadherin-associated protein) β 1 also called β-catenin is encoded by the CTNNB1 gene in humans. It is a dual function protein that regulates the coordination of cell-cell adhesion and gene transcription. It is useful for differential diagnosis of osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and provide a potential target for CCA therapy. Mutations in β-catenin are associated with ovarian carcinoma. In addition, mutations in β-catenin are linked to intellectual disability. NEK2 (Never in mitosis A-related kinase 2) binds and phosphorylates β-catenin, thereby regulating centrosome seperation. Focal adhesion protein, Kindlin-2, enhances WNT signaling by forming a transcriptional complex with β-catenin.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

法律信息

Cy is distributed under license from Amersham Biosciences Limited.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD00282178
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 CY3 conjugate
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 15B8, monoclonal
形式 buffered aqueous solution
分子量 antigen 94 kDa
species reactivity human, bovine, chicken, canine
浓度 ~1 mg/mL
technique(s) direct immunofluorescence: 1:100 using methanol/acetone-fixed bovine MDBK/MCF-7 Cell Line
同位素/亚型 IgG1
UniProt登记号 P35222
运输 wet ice
储存温度 2-8℃
Gene Information human ... CTNNB1(1499)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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