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一般描述

Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.

应用

Glutamic-Oxalacetic Transaminase from porcine heart has been used:

• in an enzyme-coupled assay for evaluating L -asparaginase enzyme activity
• in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples
• as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system

包装

10000 units in serum bottle

生化/生理作用

Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L -aspartate and α-ketoglutarate with oxalacetate and L -glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L -glutamate. Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.

单位定义

一个酶活性单位是指在pH 7.5、37 ℃下，在 L -天冬氨酸存在时，每分钟将1.0 μmole的α-酮戊二酸转化为 L -谷氨酸所需的酶量。在25 ℃下，一个单位相当于约2,000 O.D.(Karmen)单位。

外形

含 0.05M 马来酸盐和 2.5mM α-酮戊二酸盐的 3.0M (NH₄)₂SO₄中的悬浮液，pH 6.0

分析说明

由缩二脲确定的蛋白质。

基本信息

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