LY 294002-CAS 154447-36-6-Calbiochem

半岛bd体育手机客户端 说明

一般描述

A cell-permeable, potent, reversible and specific phosphatidylinositol 3-kinase inhibitor (IC₅₀= 1.4 ?M) that acts on the ATP-binding site of the enzyme. Also inhibits nonhomologous DNA end-joining (NHEJ) activity of the 460 kDa phosphatidylinositol 3-like kinase DNA-PKc_s, which is the catalytic subunit of DNA-activated protein kinase. Does not affect the activities of EGF receptor kinase, MAP kinase, PKC, Pl 4-kinase, S6 kinase, and c-Src even at 50 ?M. Promotes the nuclear accumulation of active GSK-3b. Reported to suppress the proliferation of murine embryonic stem cells. Blocks the proliferation of cultured rabbit aortic smooth muscle cells without inducing apoptosis. Shown to bind to the same site as I-BET (Cat. No. 40101050~ 10 ?M). A 10 mM (1 mg/325 ?l) solution of LY 294002 (Cat. No. 440204) in DMSO is also available.
A cell-permeable, potent, reversible, and specific phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor that acts on the ATP-binding site of the enzyme (IC₅₀= 1.4 ?M). Also inhibits nonhomologous DNA end-joining (NHED) in the 460 kDa phosphatidylinositol 3-like kinase DNA-PK_cs, which is the catalytic subunit of DNA-activated protein kinase. Does not affect the activity of EGF receptor kinase, MAP kinase, PKC, PI 4-kinase, S6 kinase, and c-src at 50 ?M. Promotes the nuclear accumulation of active GSK-3b. Reported to suppress the proliferation of murine embryonic stem cells. Blocks the proliferation of cultured rabbit aortic smooth muscle cells without inducing apoptosis. Reported to inhibit histamine secretion in RBL-2H3 cells (IC₅₀= 5 ?M).

包装

5, 10, 25 mg in Plastic ampoule

生化/生理作用

Cell permeable: yes
Target IC₅₀: 1.4 ?M against phosphatidylinositol 3-kinase
Primary Target
Phosphatidylinositol 3-kinase
Product competes with ATP.
Reversible: yes

警告

Toxicity: Standard Handling (A)

制备说明

Slight warming may be required to achieve complete solubilization.

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他说明

Dittman, A., et al. 2013.ACS Chem. Biol.9,495.
Bechard, M., and Dalton. S. 2009.Mol. Cell. Biol.29,2092.
Lianguzova, M.S. et al. 2007.Cell Biol. Int.31,330.
Baumann, P., and West, S.C. 1998.Proc. Natl. Acad. Sci. USA95,14066.
Cardone, M.H., et al. 1998.Science282,1318.
Vlahos, C.J., et al. 1995.J. Immunol.154,2413.
Yano, H., et al. 1995.Biochem. J.312,145.
Vlahos, C.J., et al. 1994.J. Biol. Chem.269,5241.



Selected Citations
Lee, J., et al. 2009. Cell Stem Cell 5,76.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C19H17NO3
分子量	307.34

半岛bd体育手机客户端 性质

质量水平	100
测定	≥98% (HPLC)
形式	solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze protect from light
颜色	off-white
溶解性	DMSO: 20 mg/mL ethanol: soluble
运输	ambient
储存温度	?20℃
InChI	1S/C19H17NO3/c21-17-13-18(20-9-11-22-12-10-20)23-19-15(7-4-8-16(17)19)14-5-2-1-3-6-14/h1-8,13H,9-12H2
InChI key	CZQHHVNHHHRRDU-UHFFFAOYSA-N

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 2
闪点(F)	487.4 °F
闪点(C)	253.0 ℃