半岛bd体育手机客户端 说明

一般描述

Tryptase is a glycoprotein released from mast cells during anaphylaxis, which performs a number of functions including catalyzing the activation of complement C3, converting prostromelysin to stromelysin (MMP-3), and cleaving fibrinogen resulting in a loss of clotting potential. Human tryptase is a major secretory protease of human lung mast cells.

应用

Tryptase has been used in a study that purified and characterized recombinant rat mast cell protease 7 expressed inPichia pastoris. Tryptase has also been used in a study to investigate drug allergies in mast cell disease.

生化/生理作用

Tryptase is a member of the serine protease S1 family. It is the predominant neutral protease of the mast cell granules. Within the mast cell granule it exists as a heparin-stabilized active tetramer. Stabilization is a result of the high negative charge density of the glycosaminoglycan. This stabilization activity is observed with heparins with a MW greater than 6 kDa as well as other glycosaminoglycans such as dextran sulfate or chondroitin sulfates. Removal of heparin results in dissociation of the tetramer and inactivation of the enzyme. High concentrations of NaCl will result in the dissociation of heparin.
Tryptase is released from the mast cell as a result of the degranulation response during anaphylaxis. In addition, several tryptase genes and alleles (α, β, γ & δ) have been identified in various tissues and circulating in blood. Pro-β-tryptase is thought to be the constituative circulating form in blood.
The biological function of tryptase is unknown. However it has been reported to catalyze the activation of complement C3, convert prostromelysin to stromelysin (MMP-3), and cleave fibrinogen resulting in a loss of clottting potential. Tryptase also degrades fibronectin, calcitonin gene-related peptide, vasoactive intestinal peptide,
and kininogen.

质量

Highly purified

物理属性

Molecular Weight: ~135 kDa (Human)(Non-covalently linked tetramer with two sets of dissimilar subunits possibly resulting from heterogeneity in N-linked glycosylation and existence of a & b isoforms sequences in human lung). 31-33 kDa (Monomer MW)

单位定义

One unit will hydrolyze 1.0 μmole of N-benzyl-DL-Arg-pNA per minute at pH 8 at 25 ℃.

外形

Supplied as a liquid in 50mM Sodium Acetate, 1M Sodium Chloride, pH 5.0 with 0.05mM Heparin and 0.01% Sodium Azide

基本信息

酶学委员会(EC)编号	3.4.21.59 ( BRENDA | IUBMB )
PubChem化学物质编号	329826858
NACRES	NA.54

半岛bd体育手机客户端 性质

形式	buffered aqueous solution
质量水平	200
specific activity	≥5 units/mg protein
分子量	~135 kDa
UniProt登记号	P20231 Q15661 Q9BZJ3 Q9NRR2
运输	dry ice
储存温度	?20℃
Gene Information	human ... TPSAB1(7177), TPSB2(64499), TPSD1(23430), TPSG1(25823)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable