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一般描述

A potent, cell-permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC₅₀= 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation ofc-Jun and blocks the expression of IL-2, IFN-γ, TNF-α, and COX-2 in cells. Blocks IL-1-induced accumulation of phospho-Jun and induction ofc-Jun transcription.
A potent, cell-permeable, selective, and reversible inhibitor ofc-Jun N-terminal kinase (JNK) (IC₅₀= 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation ofc-Jun and blocks cellular expression of IL-2, IFN-γ, TNF-α, and COX-2. Blocks IL-1-induced accumulation of phospho-Jun and induction ofc-Jun transcription. A 50 mM (5 mg/454 ?l) solution of JNK Inhibitor II (Cat. No. 420128) in DMSO is also available.

包装

5, 25, 50 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Target IC₅₀: 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3
Primary Target
JNK 1, JNK 2, JNK 3
Reversible: yes
Cell permeable: yes
Product competes with ATP.

警告

Toxicity: Standard Handling (A)

制备说明

For every 10 ?M concentration of JNK Inhibitor II, include 0.1% DMSO in the culture medium. Pre-warming of culture medium and addition of BSA to the medium may enhance its solubility.

重悬

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

其他说明

Shin, M., and Boyd, Y.D. 2002.Biochim. Biophys. Acta1589,311.
Bennett, B.L., et al. 2001.Proc. Natl. Acad. Sci. USA98,13681.
Han, Z., et al. 2001.J. Clin. Invest.108,73.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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