Glyoxalase I fromSaccharomyces cerevisiae

半岛bd体育手机客户端 说明

一般描述

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn²⁺metalloenzyme.

应用

Glyoxalase I fromSaccharomyces cerevisiaehas been used as a standard in Glyoxalase I assay forC. dilatatafemales and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

包装

500, 1000, 2500 units in glass bottle

生化/生理作用

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

单位定义

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 ℃.

外形

Solution in 50% glycerol, 0.4 M (NH₄)₂SO₄and 0.002 M KH₂PO₄pH 6.5

分析说明

Protein determined by biuret.

基本信息

酶学委员会(EC)编号	4.4.1.5 ( BRENDA | IUBMB )
MDL编号	MFCD00131225
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
类型	Grade IV
形式	buffered aqueous glycerol solution
specific activity	≥400 units/mg protein
异质活性	glyoxalase II ≤1%
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)