半岛bd体育手机客户端 说明
一般描述
Genotype:
endA1 hsdR17(rK12–mK12+)supE44 thi-1 recA1 gyrA96 relA1 lacF′[proA+B+lacIqZΔM15::Tn10] (TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
NovaBlue competent cells are for routine molecular cloning applications. NovaBlue is a K-12 strain derivative that offers high transformation efficiency,facilitates plasmid stability, and allows blue/whitescreening with appropriate plasmids.
NovaBlue Singles Competent Cells, like all Novagen Singles Competent Cells, are designed for ultimate convenience and reliability in plasmid transformation. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-l volumes that eliminate the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37℃ for 30 minutes (when selecting for kanamycin resistance).
NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.
组分
?11or22Singles Competent Cells?2or4 × 2 mlSOC Medium?10 ?lTest Plasmid
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
DNA图谱分析
Genotype:endA1 hsdR17(rK12–mK12+)supE44 thi-1 recA1 gyrA96 relA1 lacF?[proA+B+lacIqZΔM15::Tn10] (TetR)
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| manufacturer/tradename | Novagen? |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 细胞转化 | transformation efficiency: >1.5×108cfu/μg |
| 储存温度 | ?70℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
