半岛bd体育手机客户端 说明

应用

EX-CELL^?302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR^-or Glutamine Synthetase, or the GS System?, because it does not contain hypoxanthine, thymidine or L -glutamine. For use with the DHFR^-selection system we recommend the addition of 4 mM L -glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic? F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.

注意

请勿冰冻。避光保存。培养基中的几种成分不耐光，不应长时间暴露在光照下。

数量

Formulated to contain 21.2 grams of powder per liter of medium.

附注

Ex-Cell 302 has also been used successfully with BHK-21 cells and chicken embryo-related (CER) cell lines.

法律信息

EX-CELL is a registered trademark of sigma-aldrich Co. LLC

基本信息

MDL编号	MFCD01634638
NACRES	NA.71

半岛bd体育手机客户端 性质

质量水平	500
描述	for research or for further manufacturing use
无菌性	sterile-filtered
形式	dry powder
储存条件	protect from light
technique(s)	cell culture | mammalian: suitable
pH值(酸碱度)	7.1-7.7
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable