半岛bd体育手机客户端 说明

应用

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.
Achromopeptidase from bacteria may be used:

• in the homogenization step for genomic DNA extraction from microbial biofilm
• in the lysis of gram-positive strains(
• for DNA extraction fromNocardiastrains

生化/生理作用

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀= 0.6 over 2 hours at 37 ℃.
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

单位定义

One unit will produce a change in A₆₀₀of 0.001 per minute per mL at pH 8.0 at 37 ℃ using a suspension ofMicrococcus lysodeikticusas substrate (1 cm light path).

外形

Partially purified powder.

基本信息

酶学委员会(EC)编号	3.4.21.50 ( BRENDA | IUBMB )
MDL编号	MFCD00130333
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
形式	partially purified powder
specific activity	≥20,000 units/mg solid
分子量	27 kDa
异质活性	Collagenase, present
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H334
预防措施声明	P261 - P342 + P311
危险分类	Resp. Sens. 1
储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	dust mask type N95 (US), Eyeshields, Faceshields, Gloves