半岛bd体育手机客户端 说明

应用

The enzyme from Sigma has been used for the activation of trypsinogen in order to measure the activity of trypsin in hog pancreas. The study showed that antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure of weanling pigs.
Enterokinase from porcine intestine has been used in a study to report a new experimental model of the anomalous pancreatico-biliary junction. Enterokinase from porcine intestine has also been used in a study to investigate the insulinotropic region of the gastric inhibitory polypeptide.

包装

1500 units in poly bottle

生化/生理作用

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)₄-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

单位定义

One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 ℃.

基本信息

酶学委员会(EC)编号	3.4.21.9 ( BRENDA | IUBMB )
EC 号	232-761-1
MDL编号	MFCD00131020
eCl@ss	32160410
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
形式	salt-free, lyophilized powder
specific activity	≥0.5 units/mg solid
分子量	150 kDa
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)