半岛bd体育手机客户端 说明
细胞系来源
Human Caucasian B lymphocyte
细胞系描述
A non Ig-secreting, HAT sensitive, 6-thioguanine resistant, human B lymphocyte. Derived from the parent line WIL2.NS (Sigma Catalogue number. 90112121).
应用
Immortalising antigen-stimulated human B-lymphocytes by somatic hybridisation
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 5x1,000-5x10,000 cells/ml; 5% CO2; 37℃.
其他说明
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半岛bd体育手机客户端 性质
| 生物来源 | human spleen |
| 生长模式 | Suspension |
| 核型 | Not specified |
| 形态学 | Lymphoblast |
| 半岛bd体育手机客户端 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
Sigma-Aldrich
m.cnreagent.com
