SW 403

半岛bd体育手机客户端 说明

细胞系来源

Human Caucasian colon adenocarcinoma

细胞系描述

Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.

应用

Tumourigenicity studies

培养基

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm²using 0.25% trypsin/EDTA; No CO₂; 37℃. Cells do not reach confluency but will grow in small islands.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human colon
生长模式	Adherent
核型	2n = 46, triploid
形态学	Epithelial
半岛bd体育手机客户端	Carcinoembryonic antigen (CEA)
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃