SW 13

半岛bd体育手机客户端 说明

细胞系来源

Human Caucasian adrenal cortex adenocarcinoma

细胞系描述

Derived from biopsy tissue of a small cell carcinoma originating in the adrenal cortex of a 55 year old Caucasian female. Electron microscopy revealed bulb gap junctions and occasional paired cisternae of the E.R.

应用

Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 12
D7S820: 8,10
THO1: 7,8
TPOX: 8
vWA: 17,19

培养基

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin/EDTA; No CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human adrenal gland (cortex)
生长模式	Adherent
核型	2n = 46
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃