PNT1A human

半岛bd体育手机客户端 说明

细胞系来源

Human post pubertal prostate normal, immortalised with SV40

细胞系描述

Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice.

应用

PNT1A has been used to determine the variation in metabolite levels of human DU145 prostate cancer and PNT1A cells after treatment with selenomethionine (SeM) and Se-methylselenocysteine (SeMSC). It has also been used to study the effects of angiotensin II (Ang II) and relaxin 2 (RLN2) on the viability, proliferation, adhesion, migration and invasion of PNT1A cells.
Study of proliferation, oncogene activation and differentiation of adult prostatic cells and initial steps leading to transformation.

培养基

RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human prostate
生长模式	Adherent
核型	Aneuploid
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Dihydrotestosterone receptor
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?20℃