半岛bd体育手机客户端 说明
细胞系来源
Human foetal lung, SV40 transformed
细胞系描述
A clonal derivative of an SV40 transformed permanent line of MRC-5. Selection was made using X-ray induction in the presence of 2μg/ml thioguanine. The cells are HAT sensitive.
DNA图谱分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | human lung (fetal) |
生长模式 | Adherent |
核型 | Modal no. = 60-80 hperdiploid |
形态学 | Fibroblast |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | ?196℃ |
Sigma-Aldrich