KG1a

半岛bd体育手机客户端 说明

细胞系来源

Human Caucasian bone marrow acute myelogenous leukaemia

细胞系描述

Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen. KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4.

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

培养基

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 5x100,000 to 1,000,000 cells/ml. Do not culture below 100,000 cells/ml; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human bone marrow
生长模式	Suspension
核型	2n = 46, pseudodiploid
形态学	Lymphoblast
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃