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HT 1080

货号
CB_85111505
规格纯度
85111505
价格
4474.8 *本价格含增值税费
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半岛bd体育手机客户端 名称:
HT 1080
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

细胞系来源

Human fibrosarcoma

细胞系描述

Established from a fibrosarcoma arising adjacent to the acetabulum of a 35 year old caucasian male. The cells can give rise to tumours in immunosupressed NIH Swiss mice. Susceptible to infection by RNA tumour viruses including RD114 feline endogenous virus and FeLV.

应用

HT 1080 has been used to demonstrate that type I collagen induces matrix metallopeptidase 2 (MMP-2) activation. It has also been used to study the roles of Ras-related C3 botulinum toxin substrate 1 and 2 (Rac1 and Rac2) in regulating morphology, F-actin organization, adhesion, migration and chemotaxis of HT1080 cells.

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y,
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37℃.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

半岛bd体育手机客户端 性质

生物来源 human fibrosarcoma
生长模式 Adherent
核型 2n = 46, pseudodiploid
形态学 Epithelial
半岛bd体育手机客户端 Not specified
受体 Not specified
technique(s) cell culture | mammalian: suitable
相关疾病 cancer
运输 dry ice
储存温度 ?196℃

Sigma-Aldrich

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