HeLa DH

半岛bd体育手机客户端 说明

细胞系来源

Human cervix carcinoma

细胞系描述

HeLa DH cells are the wildtype that has been used for connexin-transfection assays and subsequent analysis of permeability and gap junction formation. The wildtype exhibits stable membrane potentials, very low gap junctional conductance and high stability under double whole-cell patch-clamp measurements. Gap junction plaques were not detected but using antibody directed against connexin 45 identified gap junction channels as dispersed particles in non typical gap junction areas.

应用

Transfection assays.

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cell/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human cervix
生长模式	Adherent
核型	Not specified
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃