HCT-8

半岛bd体育手机客户端 说明

细胞系来源

Human ileocecal adenocarcinoma

细胞系描述

From a 67 year old male. The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.

应用

Tumourigenicity studies

培养基

RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human intestine
生长模式	Adherent
核型	2n = 46, pseudodiploid
形态学	Epithelial
半岛bd体育手机客户端	Carcinoembryonic antigen (CEA)
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃