FM-3

半岛bd体育手机客户端 说明

细胞系描述

FM-3 (ESTDAB-007) was established from a metastatic tumour of a 70-year old female patient. The FM-3 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

DNA图谱分析

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 10
THO1: 6,9.3
TPOX: 8,11
vWA: 15,16

培养基

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) seed new cultures at 1.5-4 x10,000 cells/cm? using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37℃. Saturation density 11x10,000 cells/cm? .

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

半岛bd体育手机客户端 性质

生物来源	human skin
描述	Human melanoma, metastasis
生长模式	Adherent
形态学	Epithelial
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃