半岛bd体育手机客户端 说明
细胞系来源
Human cervix carcinoma
细胞系描述
Originally derived from normal amniotic tissue. Have been used and studied extensively including virus susceptibility, tumorigenicity and morphology. Contains HeLa marker chromosomes and exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. The cells are capable of growth in a variety of media supplemented with sera from several species. Ethnicity: Black.
应用
Virus, tumourigenicity and chromosome studies
培养基
EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin; 5% CO2; 37℃.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | human cervix |
生长模式 | Adherent |
核型 | Modal no. 71 |
形态学 | Epithelial-like |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | ?196℃ |