半岛bd体育手机客户端 说明
细胞系来源
Human B cell lymphocyte, EBV transformed
细胞系描述
Peripheral blood lymphocytes from a male Tunisian, immortalised by EBV transformation. The cell line contains the following deletions, at the homozygous state, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion I (del G1-EP1-A1-GP-G2-G4) Gm-Am genotype Gm b; -; -A2m2/Gm b ; -; -A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). The EZZ deletion (deletion I), which encompasses 160 kb involves two highly homologous regions located 13 kb downstream of IGHG3 and 13 kb downstream of IGHG4.
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37℃.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | human blood |
生长模式 | Suspension |
核型 | Not specified |
形态学 | Lymphoblast |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | ?196℃ |