半岛bd体育手机客户端 说明
细胞系来源
Human cervix carcinoma
细胞系描述
This cell line has been shown to be resistant to 8-azaguanine. The cell line was recloned in the depositor′s laboratory and is useful in transformation studies. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
应用
Transformation studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding 2-4x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | human cervix |
生长模式 | Adherent |
核型 | 2n = 55 |
形态学 | Epithelial |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | ?196℃ |