CFPAC-1

半岛bd体育手机客户端 说明

细胞系来源

Human Caucasian pancreatic adenocarcinoma

细胞系描述

CFPAC-1 is a ductal pancreatic adenocarcinoma derived by differential trypsinisation of explant cultures from a metastatic lesion in the liver of a 26 year old caucasian male with cystic fibrosis (CF). The cell line expresses cystic fibrosis trans-membrane regulator (CFTR) and ion transport properties consistent with the CF defect. CFPAC-1 cells show an epithelial morphology and polarisation with apical microvilli. Expression of cytokeratin and oncofoetal antigens characteristic of pancreatic duct cells have been described. The cells express the CF gene and show the most common CF mutation, deletion of three nucleotides result in a phenylalanine-508 deletion. CFPAC-1 cells are also tumorigenic in nude mice.

应用

Cystic fibrosis studies, genetic analysis

培养基

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human pancreas (liver metastasis)
生长模式	Adherent
核型	Hyperdiploid, (65-75 chromosomes)
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Cystic fibrosis trans-membrane regulator (CFTR)
technique(s)	cell culture | mammalian: suitable
相关疾病	metastasis
运输	dry ice
储存温度	?196℃