CESS

半岛bd体育手机客户端 说明

细胞系来源

Human lymphoblast

细胞系描述

Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2.

应用

T cell replacement factor (TRF) assays, B cell differentiation studies

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37℃.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

半岛bd体育手机客户端 性质

生物来源	human blood
生长模式	Suspension
核型	Not specified
形态学	Lymphoblast
半岛bd体育手机客户端	Surface Immunoglobulin ( Ig), MHC Class II
受体	Fc, complement
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃