ARH 77

半岛bd体育手机客户端 说明

细胞系来源

Human plasma cell leukaemia

细胞系描述

Derived from a 33 year old female with plasma cell leukaemia. The cells synthesise IgG1 (kappa-light chain) and are EBNA positive.Although the cells are reported to originate from a patient with a plasma cell leukaemia , it has now been shown that this is in fact an EBV transformed B lymphoblastoid cell line .

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8,9.3
TPOX: 8
vWA: 17

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 1-4x100,000 cells/ml; 5% CO₂; 37℃. Maximum cell density 1-2x1,000,000 cells/ml.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human blood
生长模式	Suspension
核型	Hypodiploid
形态学	Lymphoblast
半岛bd体育手机客户端	Immunoglobulin G (IgG1) (kappa)
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃