A 172

半岛bd体育手机客户端 说明

细胞系来源

Human glioblastoma

细胞系描述

Derived from a glioblastoma removed from a 53 year old male. Non-tumourigenic in anti-thymocyte serum treated NIH Swiss mice.

应用

Tumourigenicity studies

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃ (medium renewal every 2-3 days recommended).

其他说明

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半岛bd体育手机客户端 性质

生物来源	human nerve
生长模式	Adherent
核型	n = 80, over 20 marker chromosomes per cell (hypertriploid)
形态学	Not specified
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃