半岛bd体育手机客户端 说明
细胞系来源
Human placenta, SV40 transformed
细胞系描述
Established by SV40 ts30 transformation of human placental cells. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage.
GMO1
培养基
α-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 36℃. Change medium 2-3 times weekly.
其他说明
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半岛bd体育手机客户端 性质
| 生物来源 | human placenta |
| 生长模式 | Adherent |
| 核型 | Hypodiploid, modal chromosome number of 42 occuring in 34% of cells. Most cells contained 41-45 chromosomes. Cells with 44 chromosomes also occurred at a high frequency (26%). Cells having a higher ploidly occurred at 20.7%, which is very high. |
| 形态学 | Epithelial |
| 半岛bd体育手机客户端 | At 40C cells synthesize human chorionic gonadotrophin (hCG) and alkaline phosphatase. |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
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