3A sub E

半岛bd体育手机客户端 说明

细胞系来源

Human placenta (post crisis); SV40 transformed

细胞系描述

The cell line 3A sub E was derived from 3A(tPA-30-1)after the original line started to senesce and went through crisis. 3A subE has an unlimited lifespan and expresses human chorionic gonadotropin (hCG)

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 11,13
D7S820: 10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,17

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)

传代培养常规

Aspirate medium and add 0.25% trypsin/EDTA; incubate at room temperature until cells detach. Dilute with fresh medium and aliquot into new flasks at a split ratio 1:2. Renew fluid 1-2 times per week. Incubate culture at 37℃, 5% CO₂for initial 24 hour

其他说明

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半岛bd体育手机客户端 性质

生物来源	human placenta
生长模式	Adherent
核型	Not specified
形态学	Not specified
半岛bd体育手机客户端	Human chorionic gonadotropin (hCG)
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃