半岛bd体育手机客户端 说明
细胞系来源
Human skin fibroblast
细胞系描述
Established from a biopsy of a 51 year old normal male. The cells have been used in radiation survival studies.
应用
DNA repair studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
传代培养常规
Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. Allow cultures to reach confluency before splitting and do not split more than 1:4.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | human skin |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Fibroblast |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | ?196℃ |