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半岛bd体育手机客户端 说明

细胞系来源

Human skin fibroblast

细胞系描述

A thioguanine resistant derivative of MRC-5 CV1, selected with 5μg/ml 6-thioguanine.

应用

Genetic studies

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm³using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human skin
生长模式	Adherent
核型	Not specified
形态学	Fibroblast
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃