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WRL 68 (HeLa derivative)

货号
CB_89121403
规格纯度
89121403
价格
4474.8 *本价格含增值税费
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半岛bd体育手机客户端 名称:
WRL 68 (HeLa derivative)
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

细胞系来源

Human cervix carcinoma

细胞系描述

The human hepatic cell line WRL-68 exhibits a morphology similar to hepatocytes and hepatic primary cultures. Cells have been shown to secrete albumin and α-feto protein and express liver specific enzymes such as alanine amino transferase, aspartate amino transferase, gamma-glutamyl transpeptidase and alkaline phosphatase. Previously contaminated with Mycoplasma; treated and cured at ECACC. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

应用

In vitro hepatic model

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 1-5x10,000 cells/cm2using 0.25% trypsin; 5% CO2; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源 human cervix
生长模式 Adherent
核型 Not specified
形态学 Epithelial-like
半岛bd体育手机客户端 Not specified
受体 Not specified
technique(s) cell culture | mammalian: suitable
相关疾病 cancer
运输 dry ice
储存温度 ?196℃

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