半岛bd体育手机客户端 说明
细胞系来源
Human Caucasian lung squamous cell carcinoma
细胞系描述
Derived from a lung squamous cell carcinoma of a 72 year old Caucasian male. The B-lymphoblastoid cell line AGLCL (Sigma Catalogue number. 89120566) was derived from the same patient. Cells grow as large swollen aggregates, which will detach and eventually grow in suspension.
应用
Control cell line for heterozygosity studies using the AGLCL cell line
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000-2x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37℃.
其他说明
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基本信息
eCl@ss | 32190102 |
半岛bd体育手机客户端 性质
生物来源 | human lung |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Epithelial |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | ?196℃ |