IMR 32

半岛bd体育手机客户端 说明

细胞系来源

Human Caucasian neuroblastoma

细胞系描述

Established from an abdominal mass occurring in a 13 month old Caucasian male. The tumour was diagnosed as a neuroblastoma with two rare areas of orgonoid differentiation. The culture is a mixture of two morphologically distinct cell types, a small neuroblast-like cell and the other a large hyaline fibroblast. IMR 32 is partially resistant to polioviruses type 3 and highly resistant to ECHO-II. It is susceptible to Coxsackie B3.

应用

IMR 32 has been used to study the transmigration of cells through a humanin vitroblood-cerebrospinal fluid barrier (BCSFB) model.

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin; 5% CO₂; 37℃. The cells have a low plating efficiency and cell attachment can take up to 2-4 days after resuscitation. Cells easily detach. May n

其他说明

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半岛bd体育手机客户端 性质

生物来源	human nerve
生长模式	Adherent
核型	2n = 46
形态学	Fibroblast/neuroblast
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	metastasis
运输	dry ice
储存温度	?196℃