HT29 gluc C1

半岛bd体育手机客户端 说明

细胞系来源

Human Caucasian colon adenocarcinoma

细胞系描述

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

培养基

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO₃) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human colon
生长模式	Adherent
核型	Not specified
形态学	Epithelial
半岛bd体育手机客户端	Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃