半岛bd体育手机客户端 说明
一般描述
Consanguineous: No
细胞系描述
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA图谱分析
A: 3303
Cw: 1403
B: 44031
HLA DRB1: 1302
HLA DRB3: 301
HLA DQAP: 1.4
HLA DQA1: 102
HLA DQB1: 604
HLA DPB1: 401
血清学分析
HLA A: 33
HLA A ief: 33.2
HLA B: 44
HLA B ief: 44.1
Bw4/bw6: 4
HLA Dw: 19
HLA DR: 13
HLA Dw2: 26
HLA DR52: 52
HLA DQ: 6
HLA DP: 4
培养基
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
传代培养常规
Maintain cultures between 3x105- 2x106cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
半岛bd体育手机客户端 性质
| 生物来源 | human blood |
| 种族 | Japanese |
| 性别 | male |
| 生长模式 | Suspension |
| 核型 | Not specified |
| 形态学 | Lymphoblastoid |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
Sigma-Aldrich
m.cnreagent.com
