HeLa S3

半岛bd体育手机客户端 说明

细胞系来源

Human cervix carcinoma

细胞系描述

Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line. Very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition and plaque forming ability. The cells should be handled under laboratory containment level 2.

应用

HeLa S3 has been used to study the cytotoxicity of dinuclear titanium-salan complexes. It has also been used to performin vitrogenotoxicity tests (comet and micronucleus) to study the effects of occupational exposure to carcinogens on sewage workers.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

培养基

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS)

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37℃. Culture in a spinner flask. This cell line may adhere if the culture is not agitated.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human cervix
生长模式	Suspension
核型	Modal no. 68
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃