半岛bd体育手机客户端 说明
一般描述
Homozygous: Yes
Consanguineous: Yes
细胞系描述
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA图谱分析
A: 2402
Cw: 304
B: 4001
HLA DRA: 101
HLA DRB1: 9012
HLA DRB4: 103
HLA DQAP: 3.2
HLA DQA1: 302
HLA DQB1: 3032
HLA DPA1: 1
HLA DPB1: 401
血清学分析
HLA A: 24
HLA A ief: 24.1
HLA C: 10
HLA B: 60
HLA B ief: 60
Bw4/bw6: 6
HLA Dw: 23
HLA DR: 9
HLA DR53: 53
HLA DQ: 9
HLA DP: 4
培养基
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
传代培养常规
Maintain cultures between 3x105- 2x106cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
半岛bd体育手机客户端 性质
| 生物来源 | human blood |
| 种族 | Dutch |
| 性别 | female |
| 生长模式 | Suspension |
| 核型 | Not specified |
| 形态学 | Lymphoblastoid |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
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