DEM human cell line

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一般描述

Homozygous: No
Consanguineous: No

细胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA图谱分析

A: 201
Cw: 602
B: 5701
HLA DRA: 101
HLA DRB1: 0401 1602
HLA DRB4: 101
HLA DRB5: 2
HLA DRB6: 202
HLA DQA1: 0102 03
HLA DQB1: 0502 0302
HLA DPA1: 1
HLA DPB1: 0301 0401

血清学分析

HLA A: 2
HLA A ief: 2.2
HLA C: 6
HLA B: 57
HLA B ief: 57.2
Bw4/bw6: 4
HLA DR: 4 16
HLA DR51: 51
HLA DR53: 53
HLA DQ: 5 8
HLA DP: 3 4

培养基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

传代培养常规

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

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