半岛bd体育手机客户端 说明
细胞系描述
The C70 cell line was established from a 60-year old female patient with a moderately well differentiated adenocarcinoma of the sigmoid colon classified as Dukes′ stage B.
DNA图谱分析
STR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 10
D7S820: 9
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18
培养基
Iscove′s Modified Dulbecco′s Medium, + 10% Fetal Bovine Serum (FBS) + 2 mM Glutamine
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm? using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37℃.
C70 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
半岛bd体育手机客户端 性质
| 生物来源 | human colon |
| 生长模式 | adherent |
| 形态学 | epithelial |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
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