C32 (colon)

半岛bd体育手机客户端 说明

细胞系来源

Human colorectal adenocarcinoma, well differentiated, Dukes′ stage C

细胞系描述

The C32 cell line was established from a 66-year old female patient with a well differentiated adenocarcinoma of the colon classified as Dukes′ stage C.

DNA图谱分析

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 9
D5S818: 11
D7S820: 9
THO1: 8,9
TPOX: 8,9
vWA: 14,18

培养基

Iscove′s Modified Dulbecco′s Medium, + 10% FBS + 2 mM Glutamine

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 2-4x10,000 cells/cm²using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 ℃.
C32 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

半岛bd体育手机客户端 性质

生物来源	human colon
生长模式	Adherent
核型	Not specified
形态学	Epithelial
受体	Not specified