C-4I

半岛bd体育手机客户端 说明

细胞系来源

Human Caucasian Cervical carcinoma

细胞系描述

Established from a cervical carcinoma of a 41 year old Caucasian female. Cells form stratified, epithelial colonies and retain properties of squamous cell differentiation in long term culture. They are morphologically and cytogenetically distinct from C-4 II (Sigma Catalogue number. 88102701). Reported to contain human papilloma virus. Handle as potentially biohazardous material.

应用

Differentiation studies

培养基

W aymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human cervix
生长模式	Adherent
核型	2n = 46, hypodiploid
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃