A673

半岛bd体育手机客户端 说明

细胞系来源

Human Ewing sarcoma

细胞系描述

Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice.
The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status.

应用

Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 ℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human muscle
生长模式	Adherent
核型	Near Diploid
形态学	Epithelial-like
半岛bd体育手机客户端	Transforming growth factor (Tramnsforming Growth Factor (TGF)) - ?
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃