A2058

半岛bd体育手机客户端 说明

细胞系来源

Human caucasian metastatic melanoma

细胞系描述

This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human skin (lymph node metastasis)
生长模式	Adherent
核型	Not specified
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Nerve growth factor (NGF), Laminin
technique(s)	cell culture | mammalian: suitable
相关疾病	metastasis
运输	dry ice
储存温度	?196℃