半岛bd体育手机客户端 说明
细胞系来源
Human embryo kidney
细胞系描述
Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
培养基
Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.
传代培养常规
Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO2; 37℃. Cell doubling time is 30 hours.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | human kidney |
生长模式 | Suspension |
核型 | Not specified |
形态学 | Not specified |
半岛bd体育手机客户端 | Adenovirus 5 early region (E1) antigens |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | ?196℃ |