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293 GTP-AC-free

货号
CB_05011003
规格纯度
05011003
价格
4474.8 *本价格含增值税费
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半岛bd体育手机客户端 名称:
293 GTP-AC-free
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

细胞系来源

Human Embryo Kidney, serum-free

细胞系描述

The human embryo kidney cell line 293 (Sigma Catalogue number. 85120602) adapted to grow in the animal component free medium Gene Therapy Medium 3 (Sigma G9916). This cell line grows in clumps in suspension. The original 293 cell line from which this cell line was derived was transformed with sheared human Ad5 DNA.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

培养基

Gene Therapy Medium 3 (Sigma G9916) + 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

传代培养常规

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37℃ for 1-2 minutes. Tran

其他说明

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半岛bd体育手机客户端 性质

生物来源 human kidney (embryonic)
生长模式 Aggregates in suspension
核型 Not specified
形态学 Epithelial
半岛bd体育手机客户端 Not specified
受体 Not specified
technique(s) cell culture | mammalian: suitable
运输 dry ice
储存温度 ?196℃

Sigma-Aldrich

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