半岛bd体育手机客户端 说明
细胞系来源
Human bone osteosarcoma TK-
细胞系描述
Cells are thymidine kinase deficient (TK-) and resistant to BUdR.143B and HOS (Sigma Catalogue number 87070202) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+.
应用
Tumourigenicity studies
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
培养基
EMEM (EBSS) + 2mM Glutamine + 15ug/ml Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | human bone |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Not specified |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | ?196℃ |