E. coliExtract Total

半岛bd体育手机客户端 说明

一般描述

This product is an extract ofE. coliB (ATCC 11303) grown in Kornberg Minimal media at 37℃ and taken at 3/4 log growth phase. TotalE. colilipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

应用

E. coliextract total has been used for the preparation of liposomes. It has also been used to characterize the ternary complexes obtained after purification of membrane proteins in different detergents.
E. coliExtract Total has been used:

• in the preparation of small unilamellar vesicles (SUVs)
• as a reference standard in electrospray ionization tandem mass spectrometry (ESI-MS/MS)
• in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies

E. coliExtract Total is suitable:

• for solubilization of His6-tagged wtABCG2 and its steroid-binding element (SBE) mutant variants
• to prepareE. coliliposomes for liposomal content release assay
• for the determination of the phospholipid types bound to purified AdiC in DDM (n-dodecyl-β-D-maltopyranoside) and OM (n-octyl-β-D-maltopyranoside)

生化/生理作用

TheE.colilipid extract contains phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. TheE. colilipid extract serves as a standard for profiling phospholipids, aids in the identification and taxonomic grouping of any bacterial sample. It is also used for preparing supported lipid bilayers.

包装

5 mL Clear Glass Sealed Ampule (100500C-500mg)
5 mL Clear Glass Sealed Ampule (100500C-100mg)
30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100500C-1g)

半岛bd体育手机客户端 性质

包装	pkg of 2 × 20 mL (100500C-1g) pkg of 1 × 4 mL (100500C-100mg) pkg of 5 × 4 mL (100500C-500mg)
manufacturer/tradename	Avanti Polar Lipids 100500C
浓度	25 mg/mL (100500C-100mg) 25 mg/mL (100500C-1g) 25 mg/mL (100500C-500mg)
运输	dry ice
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372 - H412
预防措施声明	P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
危险分类	Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
靶器官	Central nervous system
储存分类代码	6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK	WGK 3
闪点(F)	does not flash
闪点(C)	does not flash