Sushi Peptide S3

半岛bd体育手机客户端 说明

一般描述

TheSushi Peptide S3 is a trimer of one of the high endotoxin-binding domains, Sushi 3 (or S3) within Factor C, a lipopolysaccharide (LPS)-sensitive serine protease of the horseshoe crab (Limulus Polyphemus). S3 display detergent-like properties in disrupting LPS aggregates, with specificity for palmitoyl-oleoyl-phosphatidylglycerol (POPG) resulting from electrostatic and hydrophobic forces between the peptides and the bacterial lipids. During interaction with POPG, the S3 resumes a mixture of α-helix and β-sheet structures. The unsaturated nature of POPG confers fluidity and enhances insertion of the peptides into the lipid bilayer, causing maximal disruption of the bacterial membrane. In short, peptide S3 can bind to lipopolysaccharide (LPS) and inhibit the growth of Gram-negative bacteria without affecting mammalian cells. It has been shown that endotoxin activates Factor C-based catalytic coagulation cascade resulting in the gelation of Limulus blood. This process is the basis of Limulus Amebocyte Lysate (LAL) endotoxin detection method.

外形

Lyophilized from 50 mM Tris. HCl and 100 mM NaCl (pH 8.0).

重悬

Reconstitute with water. Recommended conc: 0.1 - 1 mg/mL.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

重组	expressed inE. coli
测定	≥90% (SDS-PAGE)
形式	lyophilized powder
分子量	16.1 kDa
包装	pkg of 1 mg pkg of 100 μg pkg of 20 μg
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable