半岛bd体育手机客户端 说明

一般描述

Creatinase is a homodimer that catalyzes hydrolysis of creatine. It consists of two monomer subunit and two defined domains; N and C terminal domains. C terminal fold have both the α helices and anti-parallel β sheet within two structurally similar domains.In between these two domains, there is a sulfhydryl group that acts as active site and the activity is metal independent.

应用

Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration.It may also be used to determine the plasma creatinine level by using a centrifugal analyser.

包装

1000 units in poly bottle

生化/生理作用

Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.

物理属性

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 1.9 x 10￣²M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu⁺⁺, Hg⁺⁺, Ag⁺
Optimum pH: 8.0
Optimum temp: 40℃
pH Stability: pH 5.5 ? 9.0 (25℃, 16hr)
Thermal stability: Below 50℃ (pH 7.5, 30 min)

单位定义

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 ℃.

外形

Lyophilized powder containing sugars and EDTA as stabilizers

基本信息

酶学委员会(EC)编号	3.5.3.3 ( BRENDA | IUBMB )
EC 号	253-471-1
MDL编号	MFCD00130875
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
生物来源	bacterial (Actinobacillusspp.)
形式	lyophilized powder
specific activity	≥4 units/mg solid
分子量	~100 kDa
储存温度	?20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)